pET expression vector customized for efficient seamless cloning
نویسندگان
چکیده
منابع مشابه
OEPR Cloning: an Efficient and Seamless Cloning Strategy for Large- and Multi-Fragments
Here, an efficient cloning strategy for large DNA fragments and for simultaneous assembly of multiple DNA fragments assembly is presented. This strategy is named OEPR (based on Overlap Extension PCR and Recombination in vivo). OEPR cloning is a seamless, restriction- and ligation-independent method. The method takes advantage of both homologous recombination enzymes in E. coli and overlap PCR. ...
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Tetanus is a disease caused by tetanus toxin, a potent inhibitor for the release of inhibitory neurotransmitter in the central nervous system that causes spastic paralysis. Fragment C (52 kD) of this toxin is responsible for binding to the neuronal membrane. For this reason, and also its non toxigenic and immunogenic nature, this fragment might be ideal for new vaccine development. Presently, w...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2020
ISSN: 0736-6205,1940-9818
DOI: 10.2144/btn-2020-0101